FastQCFastQC Report
Thu 26 May 2016
SRR1660641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28382694
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8541163.009284460453261No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTGCATCTCGTATG348810.12289531078339497TruSeq Adapter, Index 14 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1187300.079.369278
AGTGGTA1204750.078.336665
GTGGTAT1203850.078.317166
GTATCAA1207500.077.893649
TGGTATC1211450.077.6823357
AAGCAGT1271600.075.023081
GCAGTGG1305250.072.834223
CAGTGGT1322800.071.697394
AGCAGTG1380100.069.367532
CAACGCA1159750.042.80518712-13
ATCAACG1175000.042.2956210-11
ACGCAGA1200650.041.38812614-15
TCAACGC1155700.040.59691612-13
AGAGTAC1214800.040.48396718-19
AACGCAG1180850.039.610914-15
GAGTACT1133750.039.5148420-21
AGTACTT1218000.039.06606720-21
TATCAAC1225150.038.41833510-11
CGCAGAG1217500.038.31622716-17
GTACTTT1206100.037.0870822-23
Produced by FastQC (version 0.11.5)