FastQCFastQC Report
Thu 26 May 2016
SRR1660640_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660640_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27219275
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8677113.187854930008239No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC513780.18875594592434958Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1188550.081.747538
GTGGTAT1198200.081.165846
AGTGGTA1204450.080.807775
TGGTATC1201400.080.7269067
GTATCAA1200950.080.683999
AAGCAGT1302050.076.088651
CAGTGGT1297950.075.263484
GCAGTGG1314050.075.228413
AGCAGTG1408200.070.427072
CAACGCA1157900.043.79622312-13
ATCAACG1181350.043.109110-11
ACGCAGA1183750.042.79725614-15
TCAACGC1154400.042.0557912-13
AGAGTAC1187500.041.89528718-19
AACGCAG1174300.041.17767314-15
GAGTACT1139250.040.4329120-21
CGCAGAG1203250.040.0600216-17
AGTACTT1215850.039.99238620-21
TATCAAC1218350.039.77454410-11
GTACTTT1204250.038.2272122-23
Produced by FastQC (version 0.11.5)