##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660640_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27219275 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19957004732859 34.0 31.0 34.0 30.0 34.0 2 32.37026272742386 34.0 31.0 34.0 30.0 34.0 3 32.39516684408383 34.0 31.0 34.0 30.0 34.0 4 35.78770242778325 37.0 35.0 37.0 35.0 37.0 5 35.78014693631627 37.0 35.0 37.0 35.0 37.0 6 35.80208418482858 37.0 35.0 37.0 35.0 37.0 7 35.75169889719693 37.0 35.0 37.0 35.0 37.0 8 35.78117719887837 37.0 35.0 37.0 35.0 37.0 9 37.47616047819055 39.0 38.0 39.0 35.0 39.0 10-11 37.37036210553 39.0 37.0 39.0 34.5 39.0 12-13 37.397366663880646 39.0 37.0 39.0 34.5 39.0 14-15 38.78606752016724 40.0 38.5 41.0 35.0 41.0 16-17 38.72221122715429 40.0 38.0 41.0 34.5 41.0 18-19 38.6033843847788 40.0 38.0 41.0 34.0 41.0 20-21 38.56254771664565 40.0 38.0 41.0 34.0 41.0 22-23 38.4316070872571 40.0 38.0 41.0 34.0 41.0 24-25 38.39374919060114 40.0 38.0 41.0 34.0 41.0 26-27 38.33037424766089 40.0 38.0 41.0 34.0 41.0 28-29 38.22762975869122 40.0 38.0 41.0 33.5 41.0 30-31 38.048848472268276 40.0 38.0 41.0 33.0 41.0 32-33 38.0415703210317 40.0 38.0 41.0 33.0 41.0 34-35 38.10100933988873 40.0 38.0 41.0 33.0 41.0 36-37 37.95830535530428 40.0 38.0 41.0 33.0 41.0 38-39 37.659184015738845 40.0 37.0 41.0 32.5 41.0 40-41 37.51033565368658 40.0 37.0 41.0 32.0 41.0 42-43 37.44440309302874 40.0 36.5 41.0 31.5 41.0 44-45 37.54955012578402 40.0 37.0 41.0 32.0 41.0 46-47 37.431158287647264 40.0 36.5 41.0 32.0 41.0 48-49 37.278885201755 40.0 36.0 41.0 31.0 41.0 50-51 36.151915839051554 38.5 34.5 40.0 29.5 40.5 52-53 36.22946333067284 38.5 35.0 40.0 30.0 40.5 54-55 36.57908752161841 39.0 35.0 41.0 29.5 41.0 56-57 36.28711262515258 39.0 35.0 41.0 28.0 41.0 58-59 36.0425444469039 39.0 35.0 41.0 28.0 41.0 60-61 35.78147002445877 39.0 35.0 41.0 27.5 41.0 62-63 35.510791378535984 38.0 35.0 40.5 27.0 41.0 64-65 35.200660763374486 37.5 34.5 40.0 26.5 41.0 66-67 34.82810581839524 37.0 34.0 40.0 26.0 41.0 68-69 34.48880152024623 36.0 34.0 39.0 26.0 41.0 70-71 34.16749459711914 36.0 34.0 39.0 26.0 41.0 72-73 33.79732502059662 35.0 33.0 38.5 25.5 40.5 74-75 33.40899437990174 35.0 33.0 37.5 25.0 40.0 76-77 33.03090192519823 35.0 33.0 37.0 24.5 39.0 78-79 32.67617116914393 35.0 33.0 36.5 24.0 39.0 80-81 32.33805986750198 35.0 32.0 36.0 23.5 38.0 82-83 32.00835716601563 35.0 32.0 35.5 23.0 37.0 84-85 31.743182101654064 35.0 32.0 35.0 21.5 37.0 86-87 31.47830904754076 35.0 31.5 35.0 20.0 36.0 88-89 31.260169567337854 35.0 31.0 35.0 20.0 36.0 90-91 31.028212672821006 34.0 31.0 35.0 20.0 36.0 92-93 30.85895926691655 34.0 31.0 35.0 19.0 35.0 94-95 30.66939220460501 34.0 30.5 35.0 19.0 35.0 96-97 30.476344465456926 34.0 30.0 35.0 18.0 35.0 98-99 30.33140787181143 34.0 30.0 35.0 18.0 35.0 100 30.194573441063365 34.0 30.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2219392717512676 1101 2 0.22415593848781157 1101 3 0.2194496789651197 1101 4 0.178877643392795 1101 5 0.17380157016228281 1101 6 0.11515450338884392 1101 7 0.14343474340821416 1101 8 0.1586326540057783 1101 9 0.19998067864491986 1101 10-11 0.24664525740984544 1101 12-13 0.23098979413078524 1101 14-15 0.24976355006827333 1101 16-17 0.2340747050312686 1101 18-19 0.23792708183942324 1101 20-21 0.2509079454333971 1101 22-23 0.23988103360520796 1101 24-25 0.20329467409369784 1101 26-27 0.20286836722229395 1101 28-29 0.17774456377990333 1101 30-31 0.22082582899795966 1101 32-33 0.17272527323043363 1101 34-35 0.15340080702258518 1101 36-37 0.14150258904810187 1101 38-39 0.1368003677673144 1101 40-41 0.09028271479486705 1101 42-43 0.12866922418324123 1101 44-45 0.14825610494873587 1101 46-47 0.13761764740783633 1101 48-49 0.14901669098236425 1101 50-51 0.03689495705105372 1101 52-53 0.1534330889473452 1101 54-55 0.19571488385451374 1101 56-57 0.19590799459282948 1101 58-59 0.13206019410658598 1101 60-61 0.14435378533709553 1101 62-63 0.11835694733706248 1101 64-65 0.08257065401397057 1101 66-67 0.10437890824481144 1101 68-69 0.07395071095550776 1101 70-71 0.04874026613782689 1101 72-73 0.010822151341464803 1101 74-75 0.05587405549894697 1101 76-77 0.07083749478827173 1101 78-79 0.0392678035334626 1101 80-81 -0.0037283494880213652 1101 82-83 -0.05430676823388936 1101 84-85 -0.07928060406148063 1101 86-87 -0.08112718297724442 1101 88-89 -0.13252833936142494 1101 90-91 -0.09687374432091289 1101 92-93 -0.15557874045395437 1101 94-95 -0.2331298411790783 1101 96-97 -0.2065886596913451 1101 98-99 -0.2617332397805221 1101 100 -0.3153041131867802 1102 1 0.202162385224959 1102 2 0.21806893231021007 1102 3 0.17832555457980703 1102 4 0.16789703280577584 1102 5 0.18415331370437826 1102 6 0.19415824549385263 1102 7 0.17177553822813962 1102 8 0.1836478004144695 1102 9 0.21435331012327197 1102 10-11 0.23729901485862825 1102 12-13 0.21301812291527256 1102 14-15 0.2111572235485042 1102 16-17 0.22202696955979917 1102 18-19 0.24830002653258987 1102 20-21 0.2767296007984754 1102 22-23 0.2404052945579096 1102 24-25 0.23442974780042647 1102 26-27 0.23091179885504687 1102 28-29 0.22787817749070882 1102 30-31 0.24787479703147142 1102 32-33 0.15677195580510528 1102 34-35 0.1559174442534541 1102 36-37 0.14529680225594888 1102 38-39 0.13342340735225378 1102 40-41 0.1528118512279022 1102 42-43 0.1735327459856819 1102 44-45 0.1277001859558311 1102 46-47 0.1826123588021673 1102 48-49 0.2387989013458025 1102 50-51 0.2021006806487904 1102 52-53 0.2787871124001242 1102 54-55 0.22699642561662614 1102 56-57 0.2373139175409804 1102 58-59 0.23864999782783514 1102 60-61 0.18130114240598516 1102 62-63 0.19042129767678517 1102 64-65 0.14796252994631942 1102 66-67 0.14526020159777886 1102 68-69 0.18226395868808254 1102 70-71 0.15092973855081482 1102 72-73 0.15438213429960967 1102 74-75 0.11441597923412417 1102 76-77 0.14556273855934165 1102 78-79 0.13467730269750433 1102 80-81 0.09745062169437091 1102 82-83 0.08404944732195929 1102 84-85 0.04409270500869056 1102 86-87 0.06811253532886141 1102 88-89 0.029699805028425175 1102 90-91 0.00539579000138346 1102 92-93 0.020425368196139715 1102 94-95 -0.0861141505530938 1102 96-97 -0.07491124017230533 1102 98-99 -0.16234039866471406 1102 100 -0.22798684004997938 1103 1 0.08099310175678909 1103 2 0.12122229473522594 1103 3 0.09845034857296753 1103 4 0.1374392838053211 1103 5 0.12447661215136918 1103 6 0.10117390453816455 1103 7 0.09834380983841129 1103 8 0.10424713433707211 1103 9 0.12223042100201553 1103 10-11 0.14342211661662674 1103 12-13 0.14230246444392236 1103 14-15 0.1370607362023719 1103 16-17 0.21137050082929676 1103 18-19 0.2073758113850488 1103 20-21 0.1913321982229519 1103 22-23 0.18261944147015896 1103 24-25 0.18243095982597168 1103 26-27 0.17832457381281586 1103 28-29 0.1528970439917927 1103 30-31 0.1953977190605798 1103 32-33 0.14838238230264977 1103 34-35 0.1344175652235151 1103 36-37 0.10944383690579684 1103 38-39 0.1057750049039754 1103 40-41 0.06080519310636845 1103 42-43 0.10311954995358974 1103 44-45 0.03582270653613051 1103 46-47 0.11141731028231305 1103 48-49 0.1457208443840159 1103 50-51 0.05674781505561555 1103 52-53 0.13863814650954964 1103 54-55 0.1943258452592076 1103 56-57 0.2655555090874344 1103 58-59 0.2255842367082579 1103 60-61 0.2286155665472407 1103 62-63 0.21834344204479095 1103 64-65 0.12986569727624442 1103 66-67 0.18216922018333292 1103 68-69 0.2486478058301529 1103 70-71 0.16029462535743733 1103 72-73 0.12445712665736863 1103 74-75 0.13432982798701687 1103 76-77 0.16183006384044774 1103 78-79 0.18587287674554887 1103 80-81 0.06457609151996024 1103 82-83 0.04842516634218441 1103 84-85 0.055654192337595276 1103 86-87 0.04302699480927075 1103 88-89 0.07243093474889761 1103 90-91 0.022648423901383552 1103 92-93 0.019281116238669682 1103 94-95 -0.02436907952975531 1103 96-97 0.037146879406751765 1103 98-99 0.014160860561734268 1103 100 -0.07836214555438303 1104 1 0.11203664222040999 1104 2 0.1392424577197886 1104 3 0.09862294947824068 1104 4 0.11780288539785033 1104 5 0.1273780016970747 1104 6 0.062104240509839315 1104 7 0.08727780281135011 1104 8 0.0947786201749139 1104 9 0.11395955846744954 1104 10-11 0.13505680843385193 1104 12-13 0.15316190472017155 1104 14-15 0.13181695809105776 1104 16-17 0.11831672795888437 1104 18-19 0.09733311328184158 1104 20-21 0.11077592781827406 1104 22-23 0.12847805970438486 1104 24-25 0.10004844320172168 1104 26-27 0.11349823174003859 1104 28-29 0.07774186727525034 1104 30-31 0.1180721381521792 1104 32-33 0.11526592329982321 1104 34-35 0.07727874062882023 1104 36-37 0.06761750072690376 1104 38-39 0.005268150857226317 1104 40-41 0.05213456533459748 1104 42-43 0.0922460341465694 1104 44-45 0.04749278881323704 1104 46-47 0.09241829688783554 1104 48-49 0.12306217825420163 1104 50-51 0.06551920423992641 1104 52-53 0.13835617213833729 1104 54-55 0.18844135441575105 1104 56-57 0.18664800758178046 1104 58-59 0.1628475750767251 1104 60-61 0.1651856171679711 1104 62-63 0.14718165859206067 1104 64-65 0.10770617893702905 1104 66-67 0.1524849595426616 1104 68-69 0.1706258289245639 1104 70-71 0.13977350810616684 1104 72-73 0.06765692831258718 1104 74-75 0.07599268560050376 1104 76-77 0.08739239486622097 1104 78-79 0.06436077698500497 1104 80-81 0.06135760934765955 1104 82-83 0.03503336591661821 1104 84-85 0.022280759898048785 1104 86-87 -0.005002250188564261 1104 88-89 -0.015309174806038328 1104 90-91 0.05286629217892269 1104 92-93 -0.013799086833628849 1104 94-95 -0.02965174953463645 1104 96-97 0.0033873439132250382 1104 98-99 -0.08394856610428647 1104 100 -0.1429687775101982 1105 1 0.06888535773990867 1105 2 0.0743473256259719 1105 3 0.08901465589950419 1105 4 0.05393914838182212 1105 5 0.0672302276451262 1105 6 0.006717042275731444 1105 7 0.0599703798052289 1105 8 0.070290395613263 1105 9 0.06174443481803138 1105 10-11 0.07463491595199656 1105 12-13 0.06398217342267998 1105 14-15 0.07152845788299089 1105 16-17 0.03085996800317048 1105 18-19 0.08787736564405435 1105 20-21 0.09653203749269323 1105 22-23 0.11217252465484506 1105 24-25 0.12452990956814602 1105 26-27 0.07420867865597103 1105 28-29 0.08582312740455222 1105 30-31 0.052003023312330754 1105 32-33 0.07295538712639171 1105 34-35 0.0908985163054723 1105 36-37 0.07619385176797522 1105 38-39 0.04643284089912214 1105 40-41 0.04113158022981622 1105 42-43 0.09179250858673527 1105 44-45 0.10307392600548582 1105 46-47 0.07420033001265125 1105 48-49 0.09217283081299854 1105 50-51 0.09736889879891919 1105 52-53 0.13608790105521962 1105 54-55 0.1328271608984224 1105 56-57 0.18008629919798125 1105 58-59 0.1103198837840722 1105 60-61 0.1976582200328565 1105 62-63 0.14396257154118786 1105 64-65 0.14916135697885835 1105 66-67 0.15651802359307965 1105 68-69 0.13331545617199225 1105 70-71 0.050545858842383495 1105 72-73 0.05289224171738738 1105 74-75 0.0831738437106253 1105 76-77 0.12144126022779034 1105 78-79 0.13187767135882922 1105 80-81 0.1292909951440322 1105 82-83 0.0738895395182908 1105 84-85 0.06769808996672921 1105 86-87 0.11137219349341265 1105 88-89 0.05809079833351305 1105 90-91 0.051861790974289335 1105 92-93 -0.0358385605471625 1105 94-95 -0.02892733310680029 1105 96-97 0.016771896066540393 1105 98-99 -0.029333815916093187 1105 100 -0.14354462060953566 1106 1 -0.014054006709201872 1106 2 -0.024265095229083045 1106 3 -0.0127498218698463 1106 4 -0.004532092174613922 1106 5 -0.01884468822666463 1106 6 -0.02473674854632435 1106 7 -0.015295874945344679 1106 8 0.003535019834373543 1106 9 -0.044421282124538664 1106 10-11 9.949151273289658E-4 1106 12-13 0.003126035650915071 1106 14-15 -0.02813433570091206 1106 16-17 -0.03781538593751321 1106 18-19 -0.06886553618199542 1106 20-21 -0.01438892784171486 1106 22-23 -0.0532123443831054 1106 24-25 -0.017787592678295994 1106 26-27 -0.033529480775477793 1106 28-29 -0.08644157831941612 1106 30-31 0.02219702060447304 1106 32-33 -0.014772318695086994 1106 34-35 -0.010682974585812133 1106 36-37 -0.10268476490562506 1106 38-39 -0.07630214530031765 1106 40-41 0.002978214603217566 1106 42-43 0.010157078681935161 1106 44-45 -0.01780096339022208 1106 46-47 -0.04322133768449277 1106 48-49 -0.03858731555767747 1106 50-51 -0.2389721799652591 1106 52-53 -0.07532126038780262 1106 54-55 0.035345352788269224 1106 56-57 0.04976771325255669 1106 58-59 0.06543164068646234 1106 60-61 0.14183829865319098 1106 62-63 0.08127209472196029 1106 64-65 0.08177495257533707 1106 66-67 0.13865840801739182 1106 68-69 0.10763479503927442 1106 70-71 0.07839766506562285 1106 72-73 -0.02950011801593888 1106 74-75 -0.07347065428298549 1106 76-77 0.012995178493873993 1106 78-79 -0.044615735661444944 1106 80-81 0.007279225864714078 1106 82-83 0.047209693886927084 1106 84-85 0.032288541422911265 1106 86-87 0.01160223367561386 1106 88-89 0.04785791661960204 1106 90-91 -0.02310365666990677 1106 92-93 -0.04215235783311755 1106 94-95 -0.005892241443422819 1106 96-97 0.013144008095547832 1106 98-99 -0.07140514062833958 1106 100 -0.09019284350029366 1107 1 -0.04301858320943808 1107 2 -0.0064544368892214266 1107 3 0.0330340596521026 1107 4 0.06001292355073673 1107 5 0.0423098854282955 1107 6 0.015047038503460897 1107 7 0.031061093642279047 1107 8 -0.0073778521344962655 1107 9 -0.030180290658755382 1107 10-11 0.027429060065166766 1107 12-13 0.007708526423336082 1107 14-15 -0.048719669198519 1107 16-17 -0.03688141904991227 1107 18-19 -0.06052514579022983 1107 20-21 0.011842898219057929 1107 22-23 -0.04003923822707378 1107 24-25 -0.022441764393740016 1107 26-27 -0.022561432819315996 1107 28-29 -0.052668139277194825 1107 30-31 -0.052276955172899875 1107 32-33 -0.09395404012309427 1107 34-35 -0.08120004250640278 1107 36-37 -0.0870046529275541 1107 38-39 -0.07580542338768481 1107 40-41 -0.09879169087089679 1107 42-43 -0.03468380991989761 1107 44-45 -0.048296006183413454 1107 46-47 -0.08539494423154537 1107 48-49 -0.12356669063452586 1107 50-51 -0.29987972737229285 1107 52-53 -0.07887912594718216 1107 54-55 0.012106538603084971 1107 56-57 0.07932048944286407 1107 58-59 0.12055475757222212 1107 60-61 0.14742508780708619 1107 62-63 0.11395801309829778 1107 64-65 0.0693370362313317 1107 66-67 0.09794668505904269 1107 68-69 0.10693475321827606 1107 70-71 0.08310692499384231 1107 72-73 0.08528741214714586 1107 74-75 0.047226498296353725 1107 76-77 0.08535306457023495 1107 78-79 0.08945787581397724 1107 80-81 0.06153641516929298 1107 82-83 0.0685635145527499 1107 84-85 -0.01182293038271709 1107 86-87 0.043561275355894935 1107 88-89 0.06967351958771317 1107 90-91 0.08636426723006707 1107 92-93 -0.024957775961766515 1107 94-95 -0.05316851656743893 1107 96-97 0.0035315434511318244 1107 98-99 -0.12694712746750625 1107 100 -0.07451060014301092 1108 1 -0.06599067301522865 1108 2 -0.06155480173008954 1108 3 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2316 54-55 -1.4068391804718416 2316 56-57 -1.5476981176480393 2316 58-59 -1.6901876980991517 2316 60-61 -1.2724845409022052 2316 62-63 -1.0574897420414828 2316 64-65 -1.177489471935381 2316 66-67 -1.2295878611696693 2316 68-69 -1.1268340592914683 2316 70-71 -0.9183440184026352 2316 72-73 -0.9788210332692131 2316 74-75 -1.0336096162242185 2316 76-77 -1.4160007568135313 2316 78-79 -1.1969996373897303 2316 80-81 -1.0558636245758635 2316 82-83 -1.3371797511725632 2316 84-85 -0.9803868676427463 2316 86-87 -0.9209844872967246 2316 88-89 -1.2962375819679934 2316 90-91 -1.5002304247874854 2316 92-93 -1.077729560928418 2316 94-95 -0.9305671290328092 2316 96-97 -1.579019922868003 2316 98-99 -1.5444090990822907 2316 100 -1.1659347560227324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 460.0 3 1161.0 4 1917.0 5 6020.0 6 5548.0 7 4026.0 8 8674.0 9 14227.0 10 17883.0 11 19691.0 12 22902.0 13 28118.0 14 34973.0 15 44598.0 16 57421.0 17 71731.0 18 81556.0 19 92169.0 20 107353.0 21 129845.0 22 164174.0 23 225861.0 24 314818.0 25 393389.0 26 407774.0 27 385983.0 28 357248.0 29 385079.0 30 451548.0 31 551520.0 32 693907.0 33 905352.0 34 1260872.0 35 1914321.0 36 3325142.0 37 6071073.0 38 6828221.0 39 1829390.0 40 3330.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.64198369185543 31.129971135272417 28.054052460090755 23.1739927127814 2 22.509619891450477 31.66616600772502 25.794542800385994 20.02967130043851 3 25.19699737993365 25.138732037225882 26.755474033724447 22.90879654911602 4 21.05726602247747 27.236045611046794 24.441522769844525 27.265165596631206 5 22.932969685488068 29.681507332762248 26.04183101500973 21.34369196673995 6 27.52968467361051 27.002990026252427 25.02983063474557 20.437494665391494 7 22.72375354293812 25.415895988790904 29.622271420025438 22.23807904824554 8 26.274484344612947 26.753479623990305 25.0769061308721 21.895129900524644 9 25.44885134271019 25.425114131311933 26.685276797156916 22.440757728820966 10-11 22.205416555544996 28.361540021271438 27.473623520743836 21.959419902439734 12-13 22.042737508133943 26.362700843799214 27.57735389478278 24.017207753284065 14-15 22.034809552708932 30.07428780768768 25.893668617708794 21.997234021894595 16-17 24.04794150406037 26.0735320256826 25.871706859822552 24.006819610434476 18-19 22.103952894645634 27.990770820054433 25.988238370202367 23.917037915097566 20-21 23.915451388071578 28.178653309292574 25.93963723183644 21.96625807079942 22-23 24.10502533232727 26.224727437869632 27.65622570317271 22.01402152663039 24-25 22.23276953979312 27.932300639008922 25.725873034044156 24.1090567871538 26-27 22.168039069246166 26.204966350801858 29.552360732762423 22.074633847189556 28-29 22.30334427426424 26.179637555206448 29.513680154057546 22.00333801647177 30-31 22.266589684998205 25.97604914520305 29.63285245286034 22.1245087169384 32-33 22.3392379297173 26.1294826852552 29.442089515664883 22.089189869362617 34-35 22.330478900390137 26.349469458643927 29.27105179159737 22.04899984936856 36-37 22.18853350923635 26.28083676583966 29.44582034444642 22.084809380477562 38-39 22.214166769423336 26.173006462067566 29.482388450662693 22.130438317846405 40-41 22.346194892737543 26.232497090489538 29.31625368927287 22.105054327500046 42-43 22.361522434233684 26.24291334181221 29.38073013604468 22.014834087909428 44-45 22.50228668921211 26.31321353845027 29.240466202399766 21.94403356993785 46-47 22.34542490500528 26.396269870558193 29.251981324718706 22.006323899717813 48-49 22.511082918260115 26.39734964485538 29.057819947105557 22.03374748977895 50-51 22.585361643029444 26.44891023885605 28.980752947609083 21.98497517050542 52-53 22.64582485102517 26.64130640391712 28.856100804534673 21.856767940523035 54-55 22.893070813542195 26.716903677321373 28.31745837471032 22.07256713442611 56-57 23.246231673636395 27.170962719995345 27.60280786972342 21.979997736644837 58-59 23.430869409092733 27.494496014225778 27.14694244506422 21.927692131617267 60-61 23.569058298553035 27.749884654039526 26.828269786323055 21.852787261084384 62-63 23.662952249036024 27.86614923631853 26.71101628079337 21.75988223385207 64-65 23.793435791154796 27.92056478172327 26.659879997159514 21.626119429962422 66-67 23.916843824774993 27.75029332881656 26.78352589833323 21.549336948075215 68-69 24.040912853292443 27.622052565129053 26.930712618140472 21.40632196343803 70-71 24.198047050495475 27.490271367538345 27.045410680466592 21.266270901499592 72-73 24.33759798475141 27.418747343409468 27.031563555503617 21.212091116335504 74-75 24.542589987410143 27.460049750952777 26.941332112864526 21.05602814877256 76-77 24.807270538976297 27.519274969330166 26.701618723862314 20.971835767831227 78-79 25.028889543742455 27.551580799161297 26.481833174588665 20.937696482507583 80-81 25.253226949922226 27.628172197838612 26.262754497532974 20.85584635470618 82-83 25.43912201634903 27.690092637051993 26.067053885189605 20.80373146140937 84-85 25.597873114115 27.685216048902543 25.945635183210765 20.77127565377169 86-87 25.741864201002134 27.69768938293364 25.846714112141868 20.713732303922363 88-89 25.91652729746402 27.67092958832111 25.735366031422153 20.67717708279272 90-91 26.0760254262281 27.609294332325174 25.664169306610834 20.65051093483589 92-93 26.185587815246897 27.624728986178166 25.62469723987607 20.56498595869887 94-95 26.27260662031268 27.662339616582727 25.56217551585329 20.502878247251306 96-97 26.34189465433478 27.64103592961562 25.51228539010497 20.504784025944637 98-99 26.434075833043646 27.6774731005989 25.488126421933487 20.400324644423964 100 26.45231891322643 27.72802700378476 25.422985408206905 20.396668674781903 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14347.0 1 10206.5 2 5336.0 3 4708.5 4 5035.5 5 5457.5 6 5907.5 7 6333.5 8 6443.0 9 6411.5 10 6501.0 11 8177.5 12 56501.5 13 70566.0 14 37941.5 15 40975.5 16 46541.5 17 51856.0 18 57419.5 19 63681.0 20 71279.0 21 82540.0 22 98839.0 23 118186.5 24 138296.5 25 161838.5 26 189145.5 27 221628.0 28 262705.5 29 304249.0 30 337440.5 31 370640.0 32 408340.0 33 442334.5 34 476812.0 35 521953.0 36 570043.5 37 605964.5 38 645198.5 39 688110.0 40 723399.5 41 766866.5 42 817106.0 43 842229.5 44 852262.5 45 876577.0 46 908571.0 47 938166.0 48 963130.0 49 983636.0 50 995540.5 51 1002217.5 52 1003560.0 53 994323.0 54 969770.0 55 929186.5 56 879237.5 57 816993.5 58 735621.5 59 636740.5 60 529000.0 61 426247.0 62 334670.0 63 255007.5 64 191398.5 65 143105.0 66 106818.0 67 81206.0 68 62315.5 69 48490.5 70 38352.0 71 30824.0 72 25648.5 73 21114.5 74 17872.0 75 15804.0 76 12325.0 77 7871.5 78 4945.5 79 3450.0 80 2395.5 81 1671.5 82 1210.5 83 870.0 84 622.0 85 462.5 86 356.5 87 263.0 88 229.5 89 203.0 90 149.0 91 124.5 92 105.0 93 79.5 94 61.0 95 42.0 96 34.5 97 28.0 98 20.5 99 17.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08107122618071201 2 0.031113980809554995 3 0.021462731832497375 4 0.015757216163913255 5 0.011760048715478278 6 0.011484508679970352 7 0.012917316864611566 8 0.0061684229282374355 9 0.0014989377931631169 10-11 0.002248406689744675 12-13 0.0056724508643231685 14-15 0.05169314759485695 16-17 0.054330984201452825 18-19 0.09059572674143598 20-21 0.1098449536220197 22-23 0.10841949317165868 24-25 0.11274179786199302 26-27 0.1110132433725733 28-29 0.10718691074615323 30-31 0.09156011686571372 32-33 0.08928599310598831 34-35 0.11166902865708216 36-37 0.11318633578594581 38-39 0.09994388167943488 40-41 0.0833582084754278 42-43 0.08324431859408453 44-45 0.08157270904533644 46-47 0.0778272014959987 48-49 0.08254077303675428 50-51 0.08557355036091153 52-53 0.09338602883434625 54-55 0.10372429096660364 56-57 0.10857012172440302 58-59 0.10312912448990652 60-61 0.09183933076836176 62-63 0.09949015908763184 64-65 0.09828329373210712 66-67 0.09484639102253825 68-69 0.09789753768239602 70-71 0.10434884838042158 72-73 0.12251795832181422 74-75 0.1332805521087538 76-77 0.12855044816586775 78-79 0.11953110433690832 80-81 0.11739291366136681 82-83 0.10443702119178414 84-85 0.10967228186643473 86-87 0.11522900591584455 88-89 0.11718166630081074 90-91 0.12332253522549738 92-93 0.12860923004010943 94-95 0.1433469480726434 96-97 0.1453087931254598 98-99 0.13790227697100677 100 0.13923956461000522 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.7219275E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.96388346718346 #Duplication Level Percentage of deduplicated Percentage of total 1 74.30678379073828 34.89735134769429 2 12.91218077676835 12.128123066147104 3 4.786702356061514 6.744063949264962 4 2.326799248323606 4.3710211499919955 5 1.3738822998402183 3.2261424113661006 6 0.8718328199610718 2.4566792975710623 7 0.6084226312574328 2.0001722687219843 8 0.4436423447244291 1.6668133902996898 9 0.3345438392055021 1.4140330091200228 >10 1.7917875877330989 15.064067220983537 >50 0.1453605042973193 4.755546885067504 >100 0.09719639988194348 7.267135723490194 >500 4.4195047493404476E-4 0.13748500659653337 >1k 3.8440571672488587E-4 0.3397767016235734 >5k 7.809003159514011E-6 0.024461632329031504 >10k+ 3.12360126379546E-5 3.507126939732415 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 867711 3.187854930008239 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 51378 0.18875594592434958 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.2107660839607227 1.1021601420317036E-5 7.715120994221926E-5 0.0 0.0 2 0.21078812716356332 1.1021601420317036E-5 7.715120994221926E-5 0.0 3.6738671401056787E-6 3 0.2107918010307034 2.2043202840634072E-5 7.715120994221926E-5 0.0 3.6738671401056787E-6 4 0.2107918010307034 3.306480426095111E-5 7.715120994221926E-5 0.0 7.347734280211357E-6 5 0.2107918010307034 5.14341399614795E-5 7.715120994221926E-5 0.0 7.347734280211357E-6 6 0.2108358874363847 6.612960852190222E-5 7.715120994221926E-5 0.0 7.347734280211357E-6 7 0.2108358874363847 6.612960852190222E-5 7.715120994221926E-5 0.0 7.347734280211357E-6 8 0.2108358874363847 6.980347566200789E-5 7.715120994221926E-5 0.0 1.4695468560422715E-5 9 0.2108395613035248 6.980347566200789E-5 8.082507708232494E-5 0.0 1.4695468560422715E-5 10-11 0.21086160450636543 7.898814351227209E-5 8.266201065237777E-5 0.0 1.4695468560422715E-5 12-13 0.2108983431777665 8.817281136253629E-5 8.44989442224306E-5 0.0 1.8369335700528393E-5 14-15 0.21097365745413865 9.36836120726948E-5 8.44989442224306E-5 0.0 1.8369335700528393E-5 16-17 0.21103795012909052 1.0470521349301184E-4 1.2674841633364593E-4 0.0 1.8369335700528393E-5 18-19 0.21110591667118245 1.3225921704380445E-4 1.524654863143857E-4 0.0 2.020626927058123E-5 20-21 0.21119960028325516 1.451177520341743E-4 1.579762870245442E-4 0.0 2.755400355079259E-5 22-23 0.21138696750740055 1.5613935345449133E-4 2.05736559845918E-4 0.0 3.122787069089827E-5 24-25 0.21198764478480783 1.689978884448612E-4 2.2226896197639356E-4 0.0 3.673867140105679E-5 26-27 0.21449138524078987 1.818564234352311E-4 2.3512749696676344E-4 0.0 3.673867140105679E-5 28-29 0.22214037662648986 1.9287802485554813E-4 2.4431216481702765E-4 0.0 3.673867140105679E-5 30-31 0.24075218755826522 2.0757349341597085E-4 2.553337662373447E-4 0.0 4.041253854116246E-5 32-33 0.2776892477848877 2.094104269860237E-4 2.645184340876089E-4 0.0 5.14341399614795E-5 34-35 0.34248340560136153 2.2594282911649922E-4 2.7002923479776736E-4 0.0 6.06188078117437E-5 36-37 0.44542883673426276 2.4063829767692195E-4 2.7921390264803157E-4 0.0 6.245574138179654E-5 38-39 0.5850431357925587 2.4982296552718617E-4 2.7921390264803157E-4 0.0 7.164040923206074E-5 40-41 0.7698110989363236 2.553337662373447E-4 2.8656163692824296E-4 1.8369335700528393E-6 7.715120994221926E-5 42-43 0.9972051055731646 2.7002923479776736E-4 2.8656163692824296E-4 3.6738671401056787E-6 8.082507708232494E-5 44-45 1.2711249656723038 2.920724376384014E-4 2.902355040683486E-4 3.6738671401056787E-6 8.082507708232494E-5 46-47 1.5971292402167214 3.1044177333892984E-4 2.939093712084543E-4 3.6738671401056787E-6 8.449894422243062E-5 48-49 1.976467044034053 3.324849761795639E-4 2.939093712084543E-4 9.184667850264197E-6 9.184667850264197E-5 50-51 2.402736663632665 3.5085431188009233E-4 2.939093712084543E-4 1.1021601420317036E-5 9.919441278285332E-5 52-53 2.869218596013303 3.637128468704622E-4 2.9574630477850717E-4 1.2858534990369875E-5 1.0654214706306469E-4 54-55 3.3776230263296876 3.747344482907792E-4 2.9758323834856E-4 1.4695468560422715E-5 1.120529477732232E-4 56-57 3.9293405132943473 3.8575604971109625E-4 2.9758323834856E-4 1.4695468560422715E-5 1.2307454919354023E-4 58-59 4.530399872884197 4.1882085397204735E-4 3.012571054886657E-4 1.6532402130475556E-5 1.2491148276359308E-4 60-61 5.168649789533337 4.408640568126814E-4 3.012571054886657E-4 1.8369335700528393E-5 1.3409615061385727E-4 62-63 5.83868967854581 4.6107032608326266E-4 3.012571054886657E-4 1.8369335700528393E-5 1.4879161917428E-4 64-65 6.531070353637267 4.794396617837911E-4 3.012571054886657E-4 2.3880136410686913E-5 1.5062855274433284E-4 66-67 7.255222631756356 4.922981967741609E-4 3.067679061988242E-4 2.571706998073975E-5 1.524654863143857E-4 68-69 8.007197105727467 5.014828646244252E-4 3.08604839768877E-4 2.939093712084543E-5 1.5613935345449133E-4 70-71 8.784508404430316 5.069936653345837E-4 3.08604839768877E-4 2.939093712084543E-5 1.6165015416464988E-4 72-73 9.582044341739447 5.25363001035112E-4 3.08604839768877E-4 2.939093712084543E-5 1.6532402130475554E-4 74-75 10.397534467762275 5.400584695955347E-4 3.1962644118919406E-4 3.122787069089827E-5 1.689978884448612E-4 76-77 11.230747328868972 5.529170045859047E-4 3.306480426095111E-4 3.306480426095111E-5 1.689978884448612E-4 78-79 12.077661877474693 5.584278052960631E-4 3.343219097496168E-4 3.673867140105679E-5 1.7450868915501976E-4 80-81 12.940353848513599 5.657755395762745E-4 4.6107032608326266E-4 3.857560497110963E-5 1.818564234352311E-4 82-83 13.818955501202733 6.025142109773313E-4 4.6290725965331554E-4 4.041253854116247E-5 1.8369335700528394E-4 84-85 14.708679786658536 6.245574138179653E-4 4.6290725965331554E-4 4.041253854116247E-5 1.855302905753368E-4 86-87 15.604104076982212 6.447636830885467E-4 4.647441932233684E-4 4.4086405681268144E-5 1.9287802485554813E-4 88 16.283920126454507 6.576222180789165E-4 4.6658112679342123E-4 4.4086405681268144E-5 1.9838882556570664E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 118855 0.0 81.74753 8 GTGGTAT 119820 0.0 81.16584 6 AGTGGTA 120445 0.0 80.80777 5 TGGTATC 120140 0.0 80.726906 7 GTATCAA 120095 0.0 80.68399 9 AAGCAGT 130205 0.0 76.08865 1 CAGTGGT 129795 0.0 75.26348 4 GCAGTGG 131405 0.0 75.22841 3 AGCAGTG 140820 0.0 70.42707 2 CAACGCA 115790 0.0 43.796223 12-13 ATCAACG 118135 0.0 43.1091 10-11 ACGCAGA 118375 0.0 42.797256 14-15 TCAACGC 115440 0.0 42.05579 12-13 AGAGTAC 118750 0.0 41.895287 18-19 AACGCAG 117430 0.0 41.177673 14-15 GAGTACT 113925 0.0 40.43291 20-21 CGCAGAG 120325 0.0 40.06002 16-17 AGTACTT 121585 0.0 39.992386 20-21 TATCAAC 121835 0.0 39.774544 10-11 GTACTTT 120425 0.0 38.22721 22-23 >>END_MODULE