FastQCFastQC Report
Thu 26 May 2016
SRR1660640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27219275
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8320413.056808089120669No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG348540.12804896530124332TruSeq Adapter, Index 20 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1121000.081.1857158
GTGGTAT1139100.080.031836
AGTGGTA1144250.079.7784045
GTATCAA1138850.079.678039
TGGTATC1143600.079.4292767
AAGCAGT1227100.075.190361
GCAGTGG1239750.074.174973
CAGTGGT1240650.073.840874
AGCAGTG1347450.068.601992
CAACGCA1090900.043.7008612-13
ATCAACG1109300.043.0035310-11
ACGCAGA1124600.042.42056314-15
AGAGTAC1131700.041.7364218-19
TCAACGC1087700.041.65199712-13
AACGCAG1109000.040.77361714-15
GAGTACT1080950.040.19154720-21
AGTACTT1159200.039.59902220-21
CGCAGAG1139300.039.58611316-17
TATCAAC1154350.039.34275410-11
GTACTTT1150200.037.679122-23
Produced by FastQC (version 0.11.5)