FastQCFastQC Report
Thu 26 May 2016
SRR1660639_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660639_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28427380
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8921933.138498869751627No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2883961.0145008087273608Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1231350.081.5385368
GTGGTAT1248100.080.4986956
AGTGGTA1251250.080.452675
GTATCAA1252550.079.962429
TGGTATC1259100.079.5573657
AAGCAGT1332400.076.473671
GCAGTGG1356550.075.022433
CAGTGGT1380800.073.253664
AGCAGTG1437400.071.1252062
CAACGCA1206850.043.8901212-13
ATCAACG1220950.043.4236310-11
ACGCAGA1244150.042.55753314-15
AGAGTAC1245300.041.81419818-19
TCAACGC1200500.041.67919512-13
AACGCAG1230500.040.63813814-15
GAGTACT1188050.040.15755520-21
CGCAGAG1254200.039.7393216-17
AGTACTT1277600.039.7058220-21
TATCAAC1268150.039.50228510-11
GCAGAGT1392400.037.75977316-17
Produced by FastQC (version 0.11.5)