FastQCFastQC Report
Thu 26 May 2016
SRR1660639_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660639_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28427380
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8044692.829909052469837No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTCATCTCGTATG2186960.7693146536895064TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACCACCTCATCTCGTATG757280.266391063826494TruSeq Adapter, Index 7 (97% over 35bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT286230.10068813939237455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1099050.079.4755558
AGTGGTA1117350.078.577625
GTGGTAT1117850.078.353316
GTATCAA1117850.077.9161459
TGGTATC1130350.077.333037
AAGCAGT1197150.073.973341
GCAGTGG1219000.072.472333
CAGTGGT1243800.070.811974
AGCAGTG1302650.068.124852
CAACGCA1061350.043.56215712-13
ATCAACG1079550.042.89739210-11
ACGCAGA1099900.042.07005714-15
TCAACGC1057600.041.10415312-13
AGAGTAC1124100.040.91859418-19
AACGCAG1084750.039.97189314-15
GAGTACT1067950.039.4075620-21
AGTACTT1151850.039.0484320-21
CGCAGAG1117400.038.78524416-17
TATCAAC1132200.038.48090410-11
GCAGAGT1261400.036.7640516-17
Produced by FastQC (version 0.11.5)