FastQCFastQC Report
Thu 26 May 2016
SRR1660638_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660638_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21591714
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7789993.607860867367917No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC593020.2746516557231167Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1045900.083.106818
AGTGGTA1063650.081.9954455
TGGTATC1063000.081.595347
GTATCAA1064900.081.451139
GTGGTAT1070350.081.3599856
AAGCAGT1124400.078.5065841
GCAGTGG1131250.077.8513
CAGTGGT1146200.076.308784
AGCAGTG1195000.074.0460362
CAACGCA1027300.044.19357712-13
ATCAACG1037650.043.81553610-11
ACGCAGA1052650.043.13055814-15
TCAACGC1023550.042.41510812-13
AGAGTAC1054050.042.34776718-19
AACGCAG1045300.041.4390214-15
GAGTACT1010900.040.90359520-21
AGTACTT1070700.040.6399920-21
CGCAGAG1068150.040.4518616-17
TATCAAC1077050.040.25110210-11
GTACTTT1061650.038.905622-23
Produced by FastQC (version 0.11.5)