FastQCFastQC Report
Thu 26 May 2016
SRR1660638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21591714
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7442143.4467573996209846No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAACATCTCGTATG373470.17296913065817748TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACACAAACATCTCGTATG237480.10998663654029504TruSeq Adapter, Index 5 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA997700.082.305938
AGTGGTA1016050.081.106225
TGGTATC1018350.080.6415567
GTATCAA1016650.080.517569
GTGGTAT1024450.080.381556
AAGCAGT1068050.077.663631
GCAGTGG1090250.076.0431753
CAGTGGT1099300.075.199144
AGCAGTG1148450.072.4309542
CAACGCA975750.043.9135712-13
ATCAACG985800.043.54215210-11
ACGCAGA1005050.042.66142714-15
AGAGTAC1012350.041.92999318-19
TCAACGC973150.041.92556412-13
AACGCAG990000.041.1170514-15
GAGTACT968200.040.59601220-21
AGTACTT1032300.040.20139320-21
CGCAGAG1019050.039.84118316-17
TATCAAC1030200.039.72926310-11
GTACTTT1016150.038.56987822-23
Produced by FastQC (version 0.11.5)