FastQCFastQC Report
Thu 26 May 2016
SRR1660637_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660637_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22879696
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7028443.071911444977241No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC788550.34465055829413116Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA971600.080.369968
GTGGTAT984150.079.4239966
AGTGGTA991050.079.0253755
TGGTATC987150.079.016697
GTATCAA990800.078.515529
AAGCAGT1060950.074.966711
GCAGTGG1058750.074.7940143
CAGTGGT1069400.073.496334
AGCAGTG1131450.070.339252
CAACGCA938100.043.5806912-13
ATCAACG951700.043.0218810-11
ACGCAGA961200.042.47130214-15
AGAGTAC965950.041.5922218-19
TCAACGC933150.041.58538412-13
AACGCAG948750.040.7813214-15
GAGTACT923700.039.95586420-21
AGTACTT984500.039.7506320-21
CGCAGAG973600.039.59215516-17
TATCAAC992900.039.1965210-11
GTACTTT978600.037.5576522-23
Produced by FastQC (version 0.11.5)