FastQCFastQC Report
Thu 26 May 2016
SRR1660637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22879696
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6412552.802725176068773No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGAAATCTCGTATG462440.20211807010023208TruSeq Adapter, Index 13 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACTGTGAAATCTCGTATG351250.1535203964248476TruSeq Adapter, Index 4 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA892500.079.53888
GTGGTAT908050.078.3992546
TGGTATC912200.077.862287
AGTGGTA915300.077.8193365
GTATCAA913400.077.626239
GCAGTGG978550.073.226353
AAGCAGT985200.072.901631
CAGTGGT993650.071.957514
AGCAGTG1054700.068.34942
CAACGCA864450.043.1961512-13
ATCAACG877250.042.65961510-11
ACGCAGA888450.042.00283414-15
AGAGTAC897800.041.1475418-19
TCAACGC860100.041.03259312-13
AACGCAG873150.040.24172614-15
GAGTACT857450.039.58057420-21
CGCAGAG897050.039.05171216-17
AGTACTT924050.039.0344620-21
TATCAAC916300.038.70822510-11
GTACTTT918800.036.79414422-23
Produced by FastQC (version 0.11.5)