FastQCFastQC Report
Thu 26 May 2016
SRR1660636_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660636_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21757085
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7341813.374445611624903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA989800.081.797068
AGTGGTA1004600.080.704325
GTATCAA1005350.080.33129
GTGGTAT1010600.080.1161046
TGGTATC1009550.079.990167
AAGCAGT1065950.077.047841
GCAGTGG1088900.075.214753
CAGTGGT1096550.074.233764
AGCAGTG1147900.071.6344152
CAACGCA965350.043.99118812-13
ATCAACG977400.043.51822310-11
ACGCAGA992850.042.70986614-15
AGAGTAC988800.042.1203718-19
TCAACGC964650.041.89869712-13
AACGCAG981300.041.0639114-15
GAGTACT945150.040.7401120-21
AGTACTT1009700.040.16686220-21
CGCAGAG1002900.040.0287516-17
TATCAAC1018800.039.62553410-11
GTACTTT1003850.038.2217522-23
Produced by FastQC (version 0.11.5)