FastQCFastQC Report
Thu 26 May 2016
SRR1660636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21757085
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6995623.2153296271076757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA940750.080.862358
AGTGGTA958500.079.600235
GTGGTAT957800.079.5897146
GTATCAA960050.079.10469
TGGTATC964250.078.8428967
AAGCAGT1014700.075.747711
GCAGTGG1037700.073.937023
CAGTGGT1057500.072.379364
AGCAGTG1094800.070.325452
CAACGCA915850.043.64951712-13
ATCAACG932250.042.9900210-11
ACGCAGA945350.042.28244814-15
TCAACGC915700.041.40635312-13
AGAGTAC954050.041.338318-19
AACGCAG935950.040.35484714-15
GAGTACT904250.040.08091420-21
AGTACTT970750.039.57199520-21
CGCAGAG959400.039.2803116-17
TATCAAC973550.039.03762410-11
GTACTTT955200.037.87610622-23
Produced by FastQC (version 0.11.5)