FastQCFastQC Report
Thu 26 May 2016
SRR1660635_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660635_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24638060
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8842353.588898638935046No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC528510.21450958395263262Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1187750.081.963788
AGTGGTA1203450.081.155745
GTGGTAT1208850.080.562296
GTATCAA1206700.080.430529
TGGTATC1211700.080.089977
AAGCAGT1269150.078.141931
GCAGTGG1294850.076.396093
CAGTGGT1313400.074.663344
AGCAGTG1356150.073.222752
CAACGCA1166050.043.90185512-13
ATCAACG1181200.043.3566110-11
ACGCAGA1199350.042.6182514-15
AGAGTAC1191650.042.10082218-19
TCAACGC1159550.041.91238412-13
AACGCAG1184750.040.921314-15
GAGTACT1133950.040.74310720-21
AGTACTT1216250.040.16108720-21
CGCAGAG1209200.039.9259916-17
TATCAAC1222450.039.65406810-11
GCAGAGT1327250.038.29788216-17
Produced by FastQC (version 0.11.5)