FastQCFastQC Report
Thu 26 May 2016
SRR1660635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24638060
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8187433.32308225566461No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACACAATCTCGTATG334520.1357736769859315TruSeq Adapter, Index 7 (97% over 37bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT246850.10019051824697238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1108600.080.734478
GTGGTAT1129500.079.436136
AGTGGTA1131550.079.41685
GTATCAA1126150.079.259319
TGGTATC1135300.078.831627
AAGCAGT1186900.076.194461
GCAGTGG1212900.074.392463
CAGTGGT1233800.072.9570854
AGCAGTG1273800.071.130932
CAACGCA1080250.043.6408612-13
ATCAACG1095500.043.09126710-11
ACGCAGA1110600.042.4609614-15
AGAGTAC1120400.041.57840718-19
TCAACGC1077850.041.29434212-13
AACGCAG1097900.040.43535214-15
GAGTACT1059150.040.3605820-21
AGTACTT1139400.039.84847320-21
CGCAGAG1126650.039.28464516-17
TATCAAC1139800.039.12207810-11
GTACTTT1126900.037.8063822-23
Produced by FastQC (version 0.11.5)