FastQCFastQC Report
Thu 26 May 2016
SRR1660634_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660634_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29591613
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9901883.3461778511364013No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC541980.18315324683382417Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1371650.080.581488
AGTGGTA1395650.079.43155
TGGTATC1392600.079.25657
GTGGTAT1396300.079.2340856
GTATCAA1402700.078.6336759
AAGCAGT1454350.077.3055341
GCAGTGG1496800.074.8571553
CAGTGGT1506500.073.891734
AGCAGTG1574100.071.531372
CAACGCA1344850.043.280412-13
ATCAACG1369550.042.5509610-11
ACGCAGA1382450.042.0646614-15
AGAGTAC1374600.041.42812318-19
TCAACGC1343550.041.01490412-13
GAGTACT1295200.040.34844220-21
AACGCAG1363250.040.31187414-15
AGTACTT1376900.040.14356620-21
CGCAGAG1396500.039.1928516-17
TATCAAC1420700.038.7859110-11
GTACTTT1363150.038.21638522-23
Produced by FastQC (version 0.11.5)