FastQCFastQC Report
Thu 26 May 2016
SRR1660634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29591613
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9441473.190589847197583No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACACGAATCTCGTATG318970.10779067704082233TruSeq Adapter, Index 23 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1316200.079.416088
AGTGGTA1342150.078.251725
GTGGTAT1342600.078.10656
TGGTATC1341400.078.008217
GTATCAA1343000.077.635379
AAGCAGT1395500.075.810681
GCAGTGG1429200.073.85383
CAGTGGT1446150.072.776964
AGCAGTG1515500.070.010992
CAACGCA1290950.042.73243312-13
ATCAACG1313700.042.06931710-11
ACGCAGA1328900.041.5156414-15
AGAGTAC1331450.040.85278318-19
TCAACGC1285450.040.47696712-13
GAGTACT1245500.039.89119720-21
AACGCAG1306650.039.70335814-15
AGTACTT1333000.039.59440620-21
CGCAGAG1347450.038.41746516-17
TATCAAC1369500.038.06312610-11
GTACTTT1313300.037.71211622-23
Produced by FastQC (version 0.11.5)