FastQCFastQC Report
Thu 26 May 2016
SRR1660633_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660633_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40330030
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12768303.165953509084918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1810300.079.465558
AGTGGTA1833000.078.690825
GTGGTAT1830700.078.6260766
GTATCAA1841350.077.940389
TGGTATC1842850.077.906157
AAGCAGT1907350.076.634721
GCAGTGG1979600.073.684093
CAGTGGT2000250.072.4109954
AGCAGTG2072950.070.651152
CAACGCA1782750.042.7821312-13
ATCAACG1805250.042.33111210-11
ACGCAGA1834050.041.59778614-15
AGAGTAC1811350.041.129818-19
TCAACGC1775200.040.4354612-13
GAGTACT1703350.039.92846720-21
AGTACTT1824700.039.563620-21
AACGCAG1818800.039.4134414-15
CGCAGAG1857750.038.44265416-17
TATCAAC1874400.038.2671110-11
GTACTTT1801850.037.6549522-23
Produced by FastQC (version 0.11.5)