FastQCFastQC Report
Thu 26 May 2016
SRR1660633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40330030
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12338723.059437347306709No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT415190.10294810095603697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1716400.078.746818
AGTGGTA1737450.078.0929645
GTGGTAT1735650.078.065666
TGGTATC1754600.076.9948967
GTATCAA1758550.076.704389
AAGCAGT1813950.075.370291
GCAGTGG1867050.073.092523
CAGTGGT1898850.071.640764
AGCAGTG1971250.069.517472
CAACGCA1687300.042.46148312-13
ATCAACG1714150.041.87176510-11
ACGCAGA1745450.041.07513814-15
AGAGTAC1740850.040.63231318-19
TCAACGC1685800.039.92920712-13
GAGTACT1633000.039.58507520-21
AGTACTT1753900.039.163620-21
AACGCAG1722750.038.96505414-15
CGCAGAG1761150.038.02340716-17
TATCAAC1789950.037.70696310-11
GTACTTT1730250.037.30325722-23
Produced by FastQC (version 0.11.5)