FastQCFastQC Report
Thu 26 May 2016
SRR1660632_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660632_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23874331
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6820232.8567208857077504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA949200.079.930098
AGTGGTA963900.078.880015
GTATCAA970550.077.889139
GTGGTAT977000.077.73446
TGGTATC976300.077.5495457
AAGCAGT1030250.074.910861
GCAGTGG1043050.073.74163
CAGTGGT1067250.071.586384
AGCAGTG1117500.069.01192
CAACGCA924750.043.22893512-13
ATCAACG938350.042.65020410-11
ACGCAGA956800.041.84585614-15
TCAACGC922450.040.9697512-13
AGAGTAC963200.040.85806718-19
AACGCAG945500.039.92186714-15
GAGTACT905800.039.6553120-21
AGTACTT977900.038.94678520-21
CGCAGAG977950.038.5125416-17
TATCAAC981850.038.44614810-11
GTACTTT962100.037.27840822-23
Produced by FastQC (version 0.11.5)