FastQCFastQC Report
Thu 26 May 2016
SRR1660632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23874331
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6754142.829038434626713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA939000.079.686078
AGTGGTA958750.078.21615
GTATCAA960300.077.776679
GTGGTAT965050.077.666546
TGGTATC963500.077.630547
AAGCAGT1014800.074.381721
GCAGTGG1031400.073.1623153
CAGTGGT1066600.070.6376654
AGCAGTG1106300.068.417132
CAACGCA915900.043.03102512-13
ATCAACG927800.042.52469310-11
ACGCAGA945850.041.68585214-15
TCAACGC913900.040.70078712-13
AGAGTAC959200.040.69724318-19
AACGCAG935650.039.68183514-15
GAGTACT903400.039.489520-21
AGTACTT977900.038.6582320-21
CGCAGAG959750.038.60950516-17
TATCAAC977050.038.2072310-11
GTACTTT956500.037.20805722-23
Produced by FastQC (version 0.11.5)