FastQCFastQC Report
Thu 26 May 2016
SRR1660631_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660631_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28175406
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10271593.645587218867405No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT282090.10011923164478978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1439900.079.468678
AGTGGTA1458800.078.639995
GTGGTAT1458650.078.3976066
TGGTATC1458150.078.289557
GTATCAA1468750.077.756269
AAGCAGT1504700.077.223111
GCAGTGG1536750.075.428783
CAGTGGT1556200.073.882524
AGCAGTG1611750.072.199832
CAACGCA1414300.042.69014412-13
ATCAACG1434600.042.15331610-11
ACGCAGA1443500.041.8020514-15
AGAGTAC1427100.041.35555318-19
AGTACTT1404550.040.7061820-21
GAGTACT1328950.040.59097320-21
TCAACGC1410100.040.53735712-13
AACGCAG1429400.039.87920414-15
CGCAGAG1454850.039.0653816-17
TATCAAC1476600.038.66113310-11
GTACTTT1396850.038.5393722-23
Produced by FastQC (version 0.11.5)