FastQCFastQC Report
Thu 26 May 2016
SRR1660631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28175406
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9869123.5027427821270796No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT288350.10234102749042907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1383950.079.138288
AGTGGTA1404400.078.287065
GTGGTAT1411450.077.832786
TGGTATC1408000.077.796547
GTATCAA1412600.077.316999
AAGCAGT1454200.076.326761
GCAGTGG1479900.074.75673
CAGTGGT1506150.073.2790764
AGCAGTG1559000.071.3344652
CAACGCA1360600.042.30319212-13
ATCAACG1381000.041.73613710-11
ACGCAGA1389600.041.4609314-15
AGAGTAC1381350.041.11395318-19
GAGTACT1282650.040.5751220-21
AGTACTT1360000.040.4566420-21
TCAACGC1355300.040.21143712-13
AACGCAG1374000.039.55985314-15
CGCAGAG1402350.038.65455216-17
GTACTTT1350700.038.35502622-23
TATCAAC1424350.038.34618810-11
Produced by FastQC (version 0.11.5)