FastQCFastQC Report
Thu 26 May 2016
SRR1660630_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660630_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33733376
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11678013.4618562933042933No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT346320.102663901768978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1617400.080.658938
AGTGGTA1652500.079.332115
GTGGTAT1652650.079.071766
GTATCAA1648400.078.8800059
TGGTATC1652000.078.847127
AAGCAGT1698100.078.04371
GCAGTGG1735450.076.227383
CAGTGGT1774600.074.1319964
AGCAGTG1819350.073.052922
CAACGCA1584650.043.35732312-13
ATCAACG1609650.042.72316410-11
ACGCAGA1622850.042.29424714-15
AGAGTAC1619000.041.59377318-19
TCAACGC1577150.041.1910812-13
GAGTACT1523600.040.5748520-21
AACGCAG1603750.040.4600314-15
AGTACTT1620150.040.30294820-21
CGCAGAG1645550.039.3154116-17
TATCAAC1672100.038.81838210-11
GTACTTT1613200.038.2885322-23
Produced by FastQC (version 0.11.5)