FastQCFastQC Report
Thu 26 May 2016
SRR1660630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33733376
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11610093.4417219314189005No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT357850.10608188163556471No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1594850.080.5648658
AGTGGTA1629550.079.094425
GTGGTAT1634550.078.80366
TGGTATC1633550.078.630347
GTATCAA1631150.078.550139
AAGCAGT1670050.077.65071
GCAGTGG1711450.075.713053
CAGTGGT1754800.073.668594
AGCAGTG1811750.071.726412
CAACGCA1561300.043.1766312-13
ATCAACG1583450.042.69729610-11
ACGCAGA1600650.042.17536514-15
AGAGTAC1608850.041.42943618-19
TCAACGC1554400.041.0767612-13
GAGTACT1510400.040.5146820-21
AACGCAG1578650.040.35497714-15
AGTACTT1612950.040.02513520-21
CGCAGAG1620000.039.2074516-17
TATCAAC1648900.038.8408810-11
GTACTTT1599900.038.1656622-23
Produced by FastQC (version 0.11.5)