FastQCFastQC Report
Thu 26 May 2016
SRR1660629_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660629_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33999539
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10024002.9482752692617393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1410950.079.151418
GTGGTAT1428100.078.266366
AGTGGTA1431750.078.207685
TGGTATC1429400.077.97087
GTATCAA1441450.077.2094659
AAGCAGT1499600.075.6635741
GCAGTGG1545500.073.224493
CAGTGGT1561750.071.983034
AGCAGTG1662500.068.381632
CAACGCA1386000.042.6601912-13
ATCAACG1402000.042.2055910-11
ACGCAGA1423650.041.504314-15
AGAGTAC1418600.041.00556618-19
TCAACGC1378500.040.40530812-13
GAGTACT1338100.039.6538620-21
AACGCAG1406400.039.49864214-15
AGTACTT1434150.039.34068720-21
CGCAGAG1441250.038.41096516-17
TATCAAC1461250.038.0759210-11
GTACTTT1410750.037.5472622-23
Produced by FastQC (version 0.11.5)