FastQCFastQC Report
Thu 26 May 2016
SRR1660629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33999539
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9846672.8961186797268046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1393550.078.398978
GTGGTAT1412650.077.531896
AGTGGTA1419250.077.4328845
TGGTATC1412350.077.372037
GTATCAA1419000.076.787589
AAGCAGT1466950.075.581451
GCAGTGG1525950.072.495763
CAGTGGT1532550.072.002664
AGCAGTG1634050.067.954762
CAACGCA1362350.042.3959312-13
ATCAACG1378700.041.85907410-11
ACGCAGA1402100.041.2716814-15
AGAGTAC1413800.040.4673718-19
TCAACGC1354700.040.11728312-13
GAGTACT1324900.039.41518820-21
AACGCAG1384100.039.22158414-15
AGTACTT1417300.039.09854520-21
CGCAGAG1421900.038.1776816-17
TATCAAC1441700.037.7941610-11
GTACTTT1382500.037.5913922-23
Produced by FastQC (version 0.11.5)