FastQCFastQC Report
Thu 26 May 2016
SRR1660628_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660628_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29138720
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9156973.142543666983313No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT329180.1129699588725929No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1341300.078.5469068
AGTGGTA1354950.077.895275
GTGGTAT1360050.077.5349356
AAGCAGT1380400.077.25351
TGGTATC1373650.076.5556647
GTATCAA1374150.076.515419
GCAGTGG1412050.075.366393
CAGTGGT1463600.072.351964
AGCAGTG1492850.071.6036452
CAACGCA1322450.042.45152312-13
ATCAACG1335800.042.09803410-11
ACGCAGA1358450.041.2664314-15
AGAGTAC1341750.040.606318-19
TCAACGC1319350.039.83113512-13
GAGTACT1249350.039.6098120-21
AGTACTT1333600.039.48569520-21
AACGCAG1342700.039.01754814-15
CGCAGAG1371850.038.0363816-17
TATCAAC1390000.037.81745510-11
GTACTTT1328850.037.17257322-23
Produced by FastQC (version 0.11.5)