FastQCFastQC Report
Thu 26 May 2016
SRR1660628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29138720
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9069763.112614418203682No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT333920.11459666038865124No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1314700.077.3083348
AGTGGTA1330750.076.697265
AAGCAGT1341050.076.507981
GTGGTAT1339100.076.110236
TGGTATC1349350.075.354477
GTATCAA1346800.075.235469
GCAGTGG1389600.073.794043
CAGTGGT1447550.070.739194
AGCAGTG1466750.070.143192
CAACGCA1295850.042.14648412-13
ATCAACG1311650.041.67103610-11
ACGCAGA1333650.040.97657814-15
AGAGTAC1320200.040.4816818-19
AGTACTT1320350.039.1479120-21
TCAACGC1289950.039.13717312-13
GAGTACT1231400.039.05027820-21
AACGCAG1314150.038.31276314-15
CGCAGAG1349650.037.25279616-17
TATCAAC1365800.037.08581510-11
GTACTTT1309150.036.55985622-23
Produced by FastQC (version 0.11.5)