FastQCFastQC Report
Thu 26 May 2016
SRR1660627_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660627_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31418896
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9523583.0311631573560063No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC951210.3027509305228293Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1321200.079.494848
GTGGTAT1336400.078.701086
AGTGGTA1341550.078.520695
TGGTATC1349400.077.682337
GTATCAA1348400.077.63759
AAGCAGT1415600.075.562541
GCAGTGG1440900.073.92743
CAGTGGT1469000.072.1004644
AGCAGTG1530200.070.027452
CAACGCA1288700.043.0459312-13
ATCAACG1308600.042.41702710-11
ACGCAGA1332900.041.5935114-15
AGAGTAC1326900.040.86235418-19
TCAACGC1285450.040.7852112-13
AACGCAG1316050.039.8057614-15
GAGTACT1252450.039.7410420-21
AGTACTT1342700.039.19050620-21
CGCAGAG1353850.038.5355216-17
TATCAAC1365900.038.34078210-11
GTACTTT1330150.037.42755522-23
Produced by FastQC (version 0.11.5)