FastQCFastQC Report
Thu 26 May 2016
SRR1660627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31418896
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8974812.8565007503764615No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCAATCTCGTATG981370.3123502493531281TruSeq Adapter, Index 16 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1268450.077.937228
AGTGGTA1285850.077.0945365
GTGGTAT1287650.076.924736
TGGTATC1294850.076.257487
GTATCAA1295250.076.070669
AAGCAGT1348750.074.145041
GCAGTGG1376200.072.388263
CAGTGGT1412400.070.42984
AGCAGTG1468300.068.1740952
CAACGCA1229400.042.63161512-13
ATCAACG1246850.042.14803310-11
ACGCAGA1274450.041.13939714-15
AGAGTAC1281400.040.36699718-19
TCAACGC1228650.039.99380512-13
GAGTACT1197300.039.21286420-21
AACGCAG1258750.038.8955914-15
AGTACTT1294000.038.78862420-21
CGCAGAG1289800.037.8171516-17
TATCAAC1311950.037.55475610-11
GTACTTT1267700.036.95139722-23
Produced by FastQC (version 0.11.5)