FastQCFastQC Report
Thu 26 May 2016
SRR1660626_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660626_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31494935
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8895112.8242985737230444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1329200.074.9692156
AAGCAGT1356650.074.694481
AGTGGTA1338900.074.6072855
GGTATCA1351750.073.615048
TGGTATC1361600.072.985067
GCAGTGG1400950.072.094173
GTATCAA1411550.070.299929
CAGTGGT1430350.070.180064
AGCAGTG1476000.068.7472462
CAACGCA1397850.037.70129812-13
AGTACTT1330450.037.3185920-21
ATCAACG1412450.037.29704310-11
GAGTACT1252450.037.20408620-21
AGAGTAC1402800.036.7825118-19
ACGCAGA1439250.036.6220314-15
TCAACGC1388450.035.7272912-13
GTACTTT1316900.035.33422522-23
AACGCAG1421100.034.8203414-15
TACTTTT1442500.034.5833922-23
TATCAAC1459600.033.955810-11
Produced by FastQC (version 0.11.5)