FastQCFastQC Report
Thu 26 May 2016
SRR1660626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31494935
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8808052.796656033740028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1330700.074.486631
GTGGTAT1322650.074.1261146
AGTGGTA1332500.073.726285
GGTATCA1346050.072.750228
TGGTATC1356600.072.1255657
GCAGTGG1384700.071.371183
GTATCAA1405550.069.503389
CAGTGGT1427900.069.0243154
AGCAGTG1467050.067.720442
CAACGCA1389900.037.33551412-13
GAGTACT1249750.037.1197720-21
AGTACTT1331200.037.10815420-21
ATCAACG1401150.037.07598510-11
AGAGTAC1396800.036.6502518-19
ACGCAGA1427700.036.35524414-15
TCAACGC1379750.035.33339712-13
GTACTTT1313800.035.21621722-23
AACGCAG1409900.034.45448714-15
TACTTTT1437250.034.43002722-23
TATCAAC1446650.033.7773710-11
Produced by FastQC (version 0.11.5)