FastQCFastQC Report
Thu 26 May 2016
SRR1660625_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660625_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38277943
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13526873.533854993200653No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850320.22214359846870557No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT442970.11572460933963981No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1767050.082.651698
AGTGGTA1784500.082.070795
GTGGTAT1787650.081.81546
GTATCAA1797950.081.038939
TGGTATC1801150.080.9827
GCAGTGG1920000.077.0700153
AAGCAGT1936550.076.696341
CAGTGGT1957600.075.0511554
AGCAGTG2034800.073.0491642
CAACGCA1711500.044.2729512-13
ATCAACG1732450.043.7954210-11
ACGCAGA1762450.042.96796814-15
TCAACGC1712850.042.53532412-13
AGAGTAC1776650.042.0678518-19
AACGCAG1740300.041.77684814-15
GAGTACT1712000.040.88729520-21
CGCAGAG1779850.040.69230316-17
TATCAAC1811400.040.1949410-11
AGTACTT1844550.039.8202720-21
GTACTTT1835100.038.1342922-23
Produced by FastQC (version 0.11.5)