FastQCFastQC Report
Thu 26 May 2016
SRR1660625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38277943
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13152193.4359709454606797No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA913680.2386962120717929No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530090.13848445304388482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1726450.082.5094768
AGTGGTA1746000.081.833225
GTGGTAT1751450.081.5061346
GTATCAA1758850.080.874679
TGGTATC1765950.080.602747
GCAGTGG1870700.076.875623
AAGCAGT1887400.076.510051
CAGTGGT1916800.074.855124
AGCAGTG1993600.072.455832
CAACGCA1662950.044.24717712-13
ATCAACG1689400.043.66707610-11
ACGCAGA1717600.042.87763614-15
TCAACGC1664200.042.5818812-13
AGAGTAC1746800.041.8434518-19
AACGCAG1695700.041.6675414-15
GAGTACT1678400.040.83995420-21
CGCAGAG1731100.040.6702416-17
TATCAAC1775350.040.0191710-11
AGTACTT1813850.039.6310820-21
GTACTTT1803500.037.97973622-23
Produced by FastQC (version 0.11.5)