FastQCFastQC Report
Thu 26 May 2016
SRR1660624_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660624_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34591676
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11812863.414942947546109No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA437120.1263656609179619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1527550.082.818768
AGTGGTA1557450.081.492985
GTGGTAT1558550.081.298196
TGGTATC1558450.081.045987
GTATCAA1560350.080.831419
AAGCAGT1689000.076.165861
CAGTGGT1681700.075.751184
GCAGTGG1696800.075.537533
AGCAGTG1845600.069.730962
CAACGCA1482450.044.51857812-13
ATCAACG1505700.043.87478310-11
ACGCAGA1518400.043.4709614-15
TCAACGC1478250.042.7007312-13
AGAGTAC1528900.042.44980618-19
AACGCAG1502100.041.94941314-15
CGCAGAG1535600.040.83651416-17
GAGTACT1484000.040.77112220-21
AGTACTT1582400.040.32339520-21
TATCAAC1567000.040.26442710-11
GAGTGTA381550.040.2538894
Produced by FastQC (version 0.11.5)