FastQCFastQC Report
Thu 26 May 2016
SRR1660624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34591676
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11443063.308038615995362No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA542370.1567920559847982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1492400.082.7916648
GTGGTAT1525350.081.2250446
AGTGGTA1528250.081.2031255
TGGTATC1523000.081.088117
GTATCAA1529450.080.564879
CAGTGGT1648300.075.616744
AAGCAGT1658100.075.610241
GCAGTGG1655450.075.58823
AGCAGTG1805050.069.5740362
CAACGCA1442350.044.4954712-13
ATCAACG1466250.043.83428210-11
ACGCAGA1480950.043.3833214-15
TCAACGC1438200.042.70919412-13
AGAGTAC1507700.042.28322218-19
AACGCAG1466150.041.78763614-15
CGCAGAG1497400.040.80100316-17
GAGTACT1461250.040.67136820-21
TATCAAC1529800.040.25325810-11
AGTACTT1558700.040.2255720-21
GTACTTT1560900.038.02759622-23
Produced by FastQC (version 0.11.5)