FastQCFastQC Report
Thu 26 May 2016
SRR1660623_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660623_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28312394
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11354174.010317884104043No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA576010.2034480023130506No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379750.13412853748785777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1455350.084.0613868
AGTGGTA1476200.083.295585
TGGTATC1475700.082.82057
GTGGTAT1485450.082.5220646
GTATCAA1483150.082.3564159
AAGCAGT1576350.078.9059751
GCAGTGG1587450.078.051463
CAGTGGT1598150.077.1969154
AGCAGTG1675800.074.228522
CAACGCA1416450.044.71932212-13
ATCAACG1436500.044.09082810-11
ACGCAGA1447150.043.76352314-15
TCAACGC1413250.043.0679312-13
AGAGTAC1465400.042.77933518-19
AACGCAG1432900.042.41931514-15
GAGTACT1416600.041.4285120-21
CGCAGAG1465850.041.3760616-17
TATCAAC1488600.040.9706710-11
AGTACTT1504850.040.85853220-21
GTACTTT1492650.039.23777822-23
Produced by FastQC (version 0.11.5)