FastQCFastQC Report
Thu 26 May 2016
SRR1660623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28312394
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9910363.500361007974105No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA642940.22708782591821802No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418750.14790342349714405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1276450.082.563148
AGTGGTA1294750.081.660695
TGGTATC1298650.081.086917
GTGGTAT1306100.080.817946
GTATCAA1306800.080.480239
AAGCAGT1383350.077.236551
GCAGTGG1401200.075.993483
CAGTGGT1418800.074.8785554
AGCAGTG1485800.072.052352
CAACGCA1236800.044.37743812-13
ATCAACG1259750.043.54845410-11
ACGCAGA1273950.043.09808714-15
TCAACGC1235450.042.46898312-13
AGAGTAC1288150.042.15970218-19
AACGCAG1256100.041.67727314-15
GAGTACT1241000.040.7757820-21
CGCAGAG1289500.040.49573516-17
AGTACTT1327300.040.09832820-21
TATCAAC1316750.039.93247210-11
GTACTTT1321350.038.26423622-23
Produced by FastQC (version 0.11.5)