FastQCFastQC Report
Thu 26 May 2016
SRR1660622_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660622_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29532482
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11279053.8192015151317116No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1377720.46651006170087567No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT519260.17582673884301359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1488800.083.629188
AGTGGTA1518150.082.1827555
GTATCAA1511700.082.12619
GTGGTAT1518200.082.119146
TGGTATC1521100.081.74477
GCAGTGG1624050.077.605453
CAGTGGT1616800.077.377524
AAGCAGT1646750.076.679881
AGCAGTG1728100.073.205362
GAGTGTA325050.044.73171694
CAACGCA1446750.044.4511312-13
ATCAACG1473800.043.73504310-11
ACGCAGA1470700.043.65352614-15
TCAACGC1445550.042.9660212-13
AGAGTAC1483550.042.75371618-19
AACGCAG1456250.042.4440914-15
GAGTACT1432300.041.55319620-21
CGCAGAG1486750.041.39995216-17
AGTACTT1526050.040.83076520-21
TATCAAC1527450.040.61182810-11
Produced by FastQC (version 0.11.5)