FastQCFastQC Report
Thu 26 May 2016
SRR1660622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29532482
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10865603.6792031228529996No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1778570.6022419653045077No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT592950.20077892538798467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1495050.083.354068
GTGGTAT1532050.081.742636
AGTGGTA1534550.081.725785
GTATCAA1523850.081.581439
TGGTATC1532800.081.4789357
GCAGTGG1627000.077.6275563
CAGTGGT1628100.077.393354
AAGCAGT1644650.076.8415151
AGCAGTG1724850.073.39552
CAACGCA1446800.044.4297212-13
ATCAACG1475550.043.6841610-11
ACGCAGA1479850.043.55434414-15
TCAACGC1441450.042.97344612-13
AGAGTAC1505100.042.49273718-19
AACGCAG1465600.042.24550214-15
GAGTACT1450350.041.4307420-21
CGCAGAG1493950.041.33081416-17
AGTACTT1535450.041.02981620-21
TATCAAC1529350.040.6624510-11
GTACTTT1547300.038.84266722-23
Produced by FastQC (version 0.11.5)