FastQCFastQC Report
Thu 26 May 2016
SRR1660621_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660621_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27036007
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9717933.594439815021501No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA412530.15258540212687471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1268300.082.787768
GTGGTAT1284550.081.8914266
AGTGGTA1292200.081.717725
TGGTATC1286350.081.563717
GTATCAA1290600.081.201769
AAGCAGT1385850.077.1538851
GCAGTGG1396350.076.304593
CAGTGGT1396300.075.893424
AGCAGTG1488150.071.919172
CAACGCA1236100.044.14644212-13
ATCAACG1250000.043.63958710-11
ACGCAGA1272050.042.91455514-15
TCAACGC1230050.042.40332412-13
AGAGTAC1287200.042.02597818-19
AACGCAG1258200.041.48553514-15
GAGTGTA268550.040.7425394
GAGTACT1238800.040.71321520-21
CGCAGAG1290450.040.2887616-17
TATCAAC1301350.040.13891610-11
AGTACTT1324150.040.06920620-21
Produced by FastQC (version 0.11.5)