FastQCFastQC Report
Thu 26 May 2016
SRR1660621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27036007
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8563593.167475877632374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA505460.18695808149480062No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285780.10570347906774843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1137850.081.3291558
AGTGGTA1159650.080.143325
GTGGTAT1157850.080.1378866
TGGTATC1158950.079.848767
GTATCAA1157300.079.832379
AAGCAGT1255350.074.831911
GCAGTGG1262050.074.202923
CAGTGGT1261650.074.0364764
AGCAGTG1358150.069.288092
CAACGCA1102250.043.74718512-13
ATCAACG1119350.043.05578210-11
ACGCAGA1137900.042.40757414-15
TCAACGC1097350.041.92986312-13
AGAGTAC1152350.041.53529718-19
AACGCAG1122700.040.8638214-15
GAGTACT1103550.040.1741120-21
CGCAGAG1155200.039.63484616-17
TATCAAC1169700.039.46290210-11
AGTACTT1195500.039.2657220-21
GTACTTT1183900.037.4614322-23
Produced by FastQC (version 0.11.5)