FastQCFastQC Report
Thu 26 May 2016
SRR1660620_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660620_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29960318
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10283853.4324902692955392No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA322910.1077792298466258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1344400.083.066888
GTGGTAT1369750.081.6392066
GTATCAA1369400.081.3485269
TGGTATC1370600.081.336157
AGTGGTA1379900.081.276635
GCAGTGG1484150.076.268353
AAGCAGT1490100.076.1680151
CAGTGGT1498600.075.116544
AGCAGTG1589000.071.4295352
CAACGCA1292100.044.68323512-13
ATCAACG1310800.044.0618610-11
ACGCAGA1319450.043.7552914-15
TCAACGC1289250.043.0828412-13
AACGCAG1312400.042.25036214-15
AGAGTAC1354650.042.133118-19
CGCAGAG1334600.041.35058616-17
GAGTACT1315100.040.75471520-21
TATCAAC1375700.040.38658510-11
AGTACTT1401050.040.12716720-21
GTACTTT1398350.038.3017122-23
Produced by FastQC (version 0.11.5)