FastQCFastQC Report
Thu 26 May 2016
SRR1660620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29960318
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8860512.9574152050055007No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA441080.14722140132157477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1245600.081.654338
GTGGTAT1271600.080.426276
TGGTATC1268150.080.314037
AGTGGTA1278250.080.191625
GTATCAA1272850.079.7179959
AAGCAGT1379400.074.818071
GCAGTGG1379650.074.750653
CAGTGGT1397600.073.666224
AGCAGTG1479800.069.840872
CAACGCA1189450.044.37407312-13
ATCAACG1209000.043.67563210-11
ACGCAGA1221950.043.21533614-15
TCAACGC1186600.042.65756212-13
AACGCAG1207850.041.84321614-15
AGAGTAC1254700.041.7465518-19
CGCAGAG1233650.040.8349216-17
GAGTACT1213800.040.35762420-21
TATCAAC1275400.039.83271410-11
AGTACTT1308000.039.39058720-21
GTACTTT1304350.037.6124522-23
Produced by FastQC (version 0.11.5)