FastQCFastQC Report
Thu 26 May 2016
SRR1660619_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660619_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32237244
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10791233.3474418594840176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA634560.19684064804050866No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371020.115090483541335No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1429900.081.94298
GTGGTAT1449050.080.978826
AGTGGTA1455900.080.8490145
TGGTATC1452650.080.56657
GTATCAA1460350.080.122289
AAGCAGT1560250.076.381351
GCAGTGG1570250.075.581573
CAGTGGT1593900.074.1158754
AGCAGTG1663300.071.651282
CAACGCA1389700.043.71085412-13
ATCAACG1403500.043.27087810-11
ACGCAGA1433800.042.32814-15
TCAACGC1383600.042.1830212-13
AACGCAG1415450.041.18332314-15
AGAGTAC1461600.041.10616718-19
GAGTACT1405450.040.1379820-21
TATCAAC1462550.039.92997410-11
CGCAGAG1457600.039.8874916-17
AGTACTT1507450.039.1553320-21
GTACTTT1488350.037.8498422-23
Produced by FastQC (version 0.11.5)