FastQCFastQC Report
Thu 26 May 2016
SRR1660619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32237244
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10306523.197084713569187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA676460.20983803702326415No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385180.11948291857703469No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1363950.081.0212868
GTGGTAT1372650.080.6915746
AGTGGTA1388350.079.873865
TGGTATC1384950.079.755937
GTATCAA1393350.079.203799
AAGCAGT1486250.075.231651
GCAGTGG1492700.074.67423
CAGTGGT1520850.073.0726554
AGCAGTG1592700.070.268922
CAACGCA1322600.043.42928312-13
ATCAACG1337500.042.92263810-11
ACGCAGA1363700.042.14623614-15
TCAACGC1317100.041.7696712-13
AACGCAG1343450.040.8804614-15
AGAGTAC1391850.040.77390318-19
GAGTACT1333450.039.67875320-21
CGCAGAG1382700.039.62824616-17
TATCAAC1400500.039.3594510-11
AGTACTT1432800.038.82800720-21
GTACTTT1419200.037.2995222-23
Produced by FastQC (version 0.11.5)