FastQCFastQC Report
Thu 26 May 2016
SRR1660618_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660618_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26078728
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7486812.8708493757824383No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA352140.13502959193408515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1009050.080.255158
AGTGGTA1028750.079.0807655
GTGGTAT1027900.078.8903666
GTATCAA1031100.078.366219
TGGTATC1035250.078.177487
AAGCAGT1128600.072.9961241
GCAGTGG1133650.072.466353
CAGTGGT1150150.071.0062264
AGCAGTG1210150.068.076522
GAGTGTA275350.045.1738194
CAACGCA971500.043.36392612-13
ATCAACG986600.042.73212410-11
ACGCAGA1008600.041.8780914-15
TCAACGC971450.041.5151812-13
AGAGTAC1021650.040.8368918-19
AACGCAG995850.040.39026314-15
GAGTACT975300.039.84567320-21
CGCAGAG1021300.039.3063416-17
TATCAAC1042600.038.7156110-11
AGTACTT1059950.038.49790220-21
Produced by FastQC (version 0.11.5)