FastQCFastQC Report
Thu 26 May 2016
SRR1660618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26078728
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6795712.6058441193910995No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA416610.15975088969063214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA968850.079.812088
AGTGGTA986950.078.850225
GTGGTAT994100.078.098666
GTATCAA988650.078.009369
TGGTATC997250.077.661937
AAGCAGT1080800.072.506761
GCAGTGG1100950.071.359663
CAGTGGT1106150.070.862834
AGCAGTG1174400.066.888642
CAACGCA932650.043.21598412-13
ATCAACG947500.042.49327510-11
ACGCAGA968150.041.65162714-15
TCAACGC934550.041.23320812-13
AGAGTAC991900.040.3605118-19
AACGCAG956500.040.1773314-15
GAGTACT945100.039.481420-21
CGCAGAG983200.039.02665716-17
TATCAAC1001350.038.50529510-11
AGTACTT1034900.037.93694720-21
GTACTTT1032650.036.2489622-23
Produced by FastQC (version 0.11.5)