FastQCFastQC Report
Thu 26 May 2016
SRR1660617_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660617_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27932392
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9358353.350357534721695No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA453160.1622345841344343No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289390.10360373003500739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1232100.082.079068
AGTGGTA1265000.080.315075
TGGTATC1266400.079.690427
GTGGTAT1272050.079.5669256
GTATCAA1269400.079.519889
AAGCAGT1349500.076.136961
GCAGTGG1372950.074.641883
CAGTGGT1373400.074.172934
AGCAGTG1451650.070.859762
CAACGCA1205650.043.976912-13
ATCAACG1223150.043.25304810-11
ACGCAGA1236500.042.87520614-15
TCAACGC1200600.041.9755112-13
AGAGTAC1243550.041.89891418-19
GAGTGTA298000.041.69951694
AACGCAG1221500.041.27972814-15
GAGTACT1194050.040.71617520-21
CGCAGAG1249700.040.2147916-17
AGTACTT1282400.039.8439920-21
TATCAAC1279500.039.4014110-11
Produced by FastQC (version 0.11.5)