FastQCFastQC Report
Thu 26 May 2016
SRR1660617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27932392
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9082563.2516227038486356No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA514240.18410166948824147No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315260.11286537866144798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1189400.081.8568958
AGTGGTA1217750.080.2715155
TGGTATC1223300.079.527597
GTGGTAT1229300.079.421326
GTATCAA1230100.078.923139
AAGCAGT1308400.075.525731
GCAGTGG1322900.074.459543
CAGTGGT1341200.073.222834
AGCAGTG1403200.070.449682
CAACGCA1161100.043.8079212-13
ATCAACG1175150.043.18018710-11
ACGCAGA1193650.042.6467714-15
TCAACGC1157600.041.89446312-13
AGAGTAC1207950.041.6634218-19
AACGCAG1173350.041.2179714-15
GAGTACT1165250.040.2946820-21
CGCAGAG1204900.040.06458716-17
AGTACTT1256850.039.36384220-21
TATCAAC1236650.039.2354610-11
GTACTTT1245300.037.67428222-23
Produced by FastQC (version 0.11.5)